The time lapse analysis reveals the reorganization of actin after treatment with Cytochalasin D: HeLa cells were subjected to confocal imaging upon treatment with 2 µM of Cytochalasin D for 1 hour and recovery for 4 hours. Actin Chromobody enables monitoring of actin dynamics in real-time in living cells.

Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14395-1-AP (ACTA2/smooth muscle actin antibody at dilution of 1:5000 (under 10x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

IP result of anti-smooth muscle actin (IP:14395-1-AP, 3ug; Detection:14395-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

1X10^6 C2C12 cell were stained with 0.2ug ACTA2/smooth muscle actin antibody (14395-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

WB result of smooth muscle actin antibody (14395-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin transfected A549 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 14395-1-AP (smooth muscle actin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 14395-1-AP (smooth muscle actin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin antibody (14395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using smooth muscle actin antibody (14395-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 smooth muscle actin antibody (CL594-14395) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite®594 smooth muscle actin antibody (CL594-14395) at dilution of 1:200, CoraLite® Plus 488 ZG16 antibody (CL488-67389, Clone: 1A7B9, green).

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 smooth muscle actin antibody (CL488-14395) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 488 smooth muscle actin antibody (CL488-14395) at dilution of 1:200, CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).

Various lysates were subjected to SDS PAGE followed by western blot with 19673-1-AP (MYH10-Specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

WB result of MYH10-Specific antibody (19673-1-AP; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYH10-Specific transfected HEK-293 cells.

Immunofluorescent analysis of HepG2 cells, using MYH10 antibody 19673-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-MYH10-Specific (IP:19673-1-AP, 4ug; Detection:19673-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 19673-1-AP (MYH10-Specific Antibody) at dilution of 1:200 (under 40x lens).

1X10^6 HepG2 cells were stained with 0.2ug MYH10-Specific antibody (19673-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYH10-Specific antibody (19673-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14869-1-AP (Calcipressin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

WB result of RCAN1 antibody (14869-1-AP, 1:1000) with si-control and si-RCAN1 transfected SKOV-3 cells.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14869-1-AP (RCAN1 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14869-1-AP (RCAN1 Antibody) at dilution of 1:200 (under 40x lens).

IP Result of anti-RCAN1 (IP:14869-1-AP, 4ug; Detection:14869-1-AP 1:500) with mouse brain tissue lysate 4000ug.

Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite® Plus 488 MYH10-Specific antibody (CL488-19673) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594-conjugated MYH10 antibody (CL594-67243, Clone: 2H8G7 ) at dilution of 1:100.

Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594-conjugated MYH10 antibody (CL594-67243, Clone: 2H8G7 ) at dilution of 1:100.